Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.1/1660
Título: Study of the antimicrobial and antioxidant effects of propolis extracts, a type of Algarve portuguese origin
Autor: Oliveira, Ana V.
Orientador: Miguel, Maria Graça
Ferreira, Ana Luísa
Palavras-chave: Própolis português
Actividade anti-bacteriana
Actividade antioxidante
Composição química
Data de Defesa: 2009
Resumo: In this work, extracts (aqueous, ethanolic and methanolic) of propolis harvested at two different times (winter and spring) from several locations of the Algarve region (B. N. Arrodeios, B. N. Pé da Serra, B. S. Arneijoafra and T. N. Madeira) were tested for their biological activities and composition. Results showed that propolis extracts were active against both Gram-positive and Gramnegative bacteria. All tested strains of bacteria showed susceptibility to the diluted propolis extracts (1:10) and in the majority of cases in a dose-dependent way. Most propolis samples collected at springtime showed higher antibacterial activity, in comparison with samples harvested at wintertime. There were also observed differences between collection sites and type of extract. These results correlate to HPLC results, where the same differences where observed. Regarding propolis citotoxicity, results showed that aqueous propolis extracts have no effect and ethanolic propolis extracts causes a small decrease in cell viability. Concerning antioxidant enzymatic activities, the superoxide dismutase (SOD), catalase (CAT) and guaiacol peroxidase (GP) activities of samples of propolis were determined. Results showed that SOD activity was dependent on the collection time and decreased drastically from winter to spring in samples from B.N. Arrodeios and B.S. Arneijoafra. The opposite was observed in samples from B.N. Pé da Serra and T.N. Madeira. We expected that the samples with higher SOD activities would have higher CAT activities also. Such was not observed, which may suggest that there could be other antioxidant enzymes involved, different to the ones tested. In respect with protein contents, major differences were not observed when comparing samples collected at different times, except for samples from B.S. Arneijoafra, where a decrease occurred from the sample collected at winter to sample collected at spring time. This was the first study of the biological activities of Portuguese propolis from the Algarve region.
Descrição: Dissertação de mest., Ciências Biomédicas, Departamento de Ciências Biomédicas e Medicina, Univ. do Algarve, 2009
Peer review: yes
URI: http://hdl.handle.net/10400.1/1660
Designação: Mestrado em Ciências Biomédicas
Aparece nas colecções:UA01-Teses

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