Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.1/1853
Título: Omega-3 fatty acid desaturase genes isolated from Purslane (Portulaca oleracea L.): expression in different tissues and response to cold and wound stress
Autor: Teixeira, Monica
Carvalho, Isabel Saraiva de
Brodelius, Maria
Palavras-chave: Portulaca oleracea
Omega-3 fatty acid desaturase
FAD7
FAD8
Cold stress
Wounding
Data: Fev-2010
Editora: ACS Publications
Resumo: Two full-length cDNA clones PoleFAD7 and PoleFAD8, encoding plastidial omega-3 fatty acid desaturases were isolated from purslane (Portulaca oleracea). The encoded enzymes convert linoleic to alpha-linolenic acid (C18:3n-3). Three histidine clusters characteristic of fatty acid desaturases, a putative chloroplast transit peptide in the N-terminal, and three putative transmembrane domains were identified in the sequence. Both genes were expressed in all analyzed tissues showing different levels of expression. PoleFAD7 was up-regulated by wounding but not by low temperature. PoleFAD8 was up-regulated by cold stress but not by wounding. Total fatty acid and linolenic acid content were higher both, in wounded and intact leaves of plants exposed to low temperature.
Peer review: yes
URI: http://hdl.handle.net/10400.1/1853
Versão do Editor: http://Www.Ncbi.Nlm.Nih.Gov/Pubmed/20070085
Aparece nas colecções:FCT2-Artigos (em revistas ou actas indexadas)

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