Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.1/3041
Título: Is it possible to improve biocontrol agents to practical applications? the pantoea agglomerans CPA-2 example
Autor: Teixidó, Neus
Cañamás, Teresa Paula
Torres, Rosario
Usall, Josep
Viñas, Inma
Palavras-chave: Biological control
Citrus
Fluidized bed drying
Improving environmental stress resistance
Pre-harvest treatments
Spray drying
Data: 2010
Editora: Universidade do Algarve
Resumo: A major hurdle in exploitation of biocontrol agents is the limited tolerance of fluctuating environmental conditions practically and the difficulties in developing a shelf-stable formulated product as effective as fresh cells. Most of microorganisms are very sensitive to drying processes involved in formulation and biological control is usually limited by the narrow range of conditions below microorganisms are able to survive, establish and effectively control pests and diseases. P. agglomerans cells grown at low water activities using NaCl exhibited osmotic adaptation and also demonstrated thermotolerance and desiccation tolerance after spray drying, freeze drying and fluidized bed drying. Different formulation strategies of P. agglomerans cells were tested in order to improve survival under field conditions and efficacy in controlling postharvest rots, including lyophilised cells, osmotic adaptation by NaCl treatments and additives. In general, osmotic adapted and lyophilised P. agglomerans cells showed greater survival rates than non-osmotic adapted or fresh cells when these bacterial treatments were sprayed at field conditions. However, this superiority was only found when additive Fungicover was added to suspension treatments. The improved formulation of P. agglomerans provided an effective control for oranges against natural postharvest pathogens infections and P. digitatum artificial infections. These results allowed us to conclude that it is possible to improve environmental stress tolerance and ecological competence of P. agglomerans by integrating certain formulation strategies. Enhancing stress tolerance and formulation strategies could be appropriate approaches to obtain consistency and broaden the spectrum of use of biocontrol agents.
Descrição: Proceedings of the International Conference “Environmentally friendly and safe technologies for quality of fruit and vegetables”, held in Universidade do Algarve, Faro, Portugal, on January 14-16, 2009. This Conference was a join activity with COST Action 924.
Peer review: yes
URI: http://hdl.handle.net/10400.1/3041
Aparece nas colecções:UED01-Edições UAlg

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