Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.1/3863
Título: CYP27A1 expression in gilthead sea bream (Sparus auratus, L.): effects of calcitriol and parathyroid hormone-related protein
Autor: Bevelander, G. S.
Pinto, E. S. L. C.
Canario, Adelino V. M.
Spanings, T.
Flik, G.
Palavras-chave: D metabolism
Fishes
Data: 2008
Editora: Society for Endocrinology
Citação: Bevelander, G. S; Pinto, E. S L C; Canario, A. V M; Spanings, T.; Flik, G. CYP27A1 expression in gilthead sea bream (Sparus auratus, L.): effects of calcitriol and parathyroid hormone-related protein, Journal of Endocrinology, 196, 3, 625-635, 2008.
Resumo: Little is known about vitamin D metabolism in fishes. Several reports have shown hydroxylase activities in various organs to produce vitamin D metabolites, but the enzymes involved have not been isolated or characterized. We isolated and characterized a renal mitochondrial hydroxylase, CYP27A1, that governs vitamin D metabolism in gilthead sea bream, Sparus auratus. The enzyme is highly expressed in kidney and to a far lesser extent in liver. When treated with 25-hydroxy vitamin D or calcitriol, the kidney responded differentially and time dependently with CYP27A1 mRNA expression levels. This response substantiates a role for CYP27A1 in fish vitamin D metabolism. This notion is strengthened by upregulation of CYP27A1 in sea bream treated with parathyroid hormone-related protein (PTHrP), and suggests an original role for PTHrP in calcitriol-regulated processes n fish similar to the role of PTH in mammalian vitamin D-dependent processes.
Peer review: yes
URI: http://hdl.handle.net/10400.1/3863
DOI: http://dx.doi.org/10.1677/JOE-07-0566
ISSN: 0022-0795
Aparece nas colecções:CCM2-Artigos (em revistas ou actas indexadas)



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