Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.1/4184
Título: Matrix gla protein in turbot (Scophthalmus maximus): gene expression analysis and identification of sites of protein accumulation
Autor: Roberto, V.
Cavaco, S.
Viegas, C. S. B.
Simes, Dina
Ortiz-Delgado, J. B.
Sarasquete, C.
Gavaia, Paulo J.
Cancela, Leonor
Palavras-chave: Turbot Mgp
Gene expression
Soft tissue accumulation
Heart
Vertebrae
Branchial arches
Data: 2009
Editora: Elsevier
Citação: Roberto, V.P.; Cavaco, S.; Viegas, C.S.B.; Simes, D.C.; Ortiz-Delgado, J.-B.; Sarasquete, M.C.; Gavaia, P.J.; Cancela, M.L. Matrix Gla protein in turbot (Scophthalmus maximus): Gene expression analysis and identification of sites of protein accumulation, Aquaculture, 294, 3-4, 202-211, 2009.
Resumo: Matrix Gla protein (Mgp) is a secreted vitamin K-dependent extracellular matrix protein and a physiological inhibitor of calcification whose gene structure, amino acid sequence and tissue distribution have been conserved throughout evolution. In the present work, the turbot (Scophthalmus maximus) mgp cDNA was cloned and the sequence of the deduced protein compared to that of other vertebrates. As expected, it was closer to teleosts than to other vertebrate groups but there was a strict conservation of amino-acids thought to be important for protein function. Analysis of mgp gene expression indicated branchial arches as the site with higher levels of expression, followed by heart, vertebra and kidney. These results were confirmed by in situ hybridization with a strong mgp expression in branchial arch chondrocytes. Mgp was found to accumulate in gills where it appeared to be restricted to chondrocytes from branchial filaments, while in vertebrae it was localized in vertebral end plates, in growth zones, in vertebral arches and spines and in notochord cells. In the soft tissues analysed, Mgp was mainly detected in kidney and heart, consistent with previous data and providing further evidence for a role of Mgp as a calcification inhibitor and a modulator of the mineralization process. Our studies provide evidence that turbot, an important new species for aquaculture, is also a useful model to study function and expression of Mgp.
Peer review: yes
URI: http://hdl.handle.net/10400.1/4184
DOI: http://dx.doi.org/10.1016/j.aquaculture.2009.06.020
ISSN: 0044-8486
Versão do Editor: http://www.sciencedirect.com/science/article/pii/S0044848609005353
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