Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.1/4193
Título: Detection of mineralized structures in very early stages of development of Marine Teleostei using a modified Alcian blue-Alizarin red double staining technique for bone and cartilage
Autor: Gavaia, Paulo J.
Sarasquete, C.
Cancela, Leonor
Palavras-chave: Bone
Alizarin red
Alcian blue
Diplodus sp.
Cartilage
Halobatrachus didactilus
Larvae
Skeleton
Sotea senegalensis
Sparus aurata
Data: 2000
Editora: Informa Healthcare
Citação: Gavaia, Paulo Jorge Travessa; Carmen Sarasquete; Leonor Cancela. Detection of mineralized structures in very early stages of development of Marine Teleostei using a modified Alcian blue-Alizarin red double staining technique for bone and cartilage, Biotechnic & Histochemistry , 75 , 79-84, 2000.
Resumo: We have developed a procedure for staining cartilage and bone in fish larvae as small as 2 mm (notochord length), for which standard alcian blue/alizarin red procedures did not give positive and/or consistent results. Small calcified structures only 100-200 pm in length can be clearly visualized. The method is suitable for both ontogenic studies during early stages of skeletal development in most marine fishes (e.g., Sparus aurata L., Solea senegalensis Kaup), whose larvae at hatching are often only a few millimeters long and for detecting skeletal abnormalities in small larvae. This procedure can also be used for specimens that have been preserved in 1000/0 ethanol for up to two years.
Peer review: yes
URI: http://hdl.handle.net/10400.1/4193
DOI: http://dx.doi.org/10.3109/10520290009064151
ISSN: 1052-0295
Aparece nas colecções:CCM2-Artigos (em revistas ou actas indexadas)



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