Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.1/4282
Título: Evolution of matrix and bone gamma-carboxyglutamic acid proteins in vertebrates
Autor: Laizé, Vincent
Martel, Paulo
Viegas, C. S. D.
Price, P. A.
Cancela, Leonor
Palavras-chave: Carboxyglutamic Acid Proteins
Data: 2005
Editora: American Society for Biochemistry and Molecular Biology
Citação: Laizé V; Martel P; Viegas CSB; Price PA; Cancela ML. Evolution of matrix and bone gamma-carboxyglutamic acid proteins in vertebrates, Journal of Biological Chemistry, 280, 29, 26659-26668, 2005.
Resumo: The evolution of calcified tissues is a defining feature in vertebrate evolution. Investigating the evolution of proteins involved in tissue calcification should help elucidate how calcified tissues have evolved. The purpose of this study was to collect and compare sequences of matrix and bone γ-carboxyglutamic acid proteins (MGP and BGP, respectively) to identify common features and determine the evolutionary relationship between MGP and BGP. Thirteen cDNAs and genes were cloned using standard methods or reconstructed through the use of comparative genomics and data mining. These sequences were compared with available annotated sequences (a total of 48 complete or nearly complete sequences, 28 BGPs and 20 MGPs) have been identified across 32 different species (representing most classes of vertebrates), and evolutionarily conserved features in both MGP and BGP were analyzed using bioinformatic tools and the Tree-Puzzle software. We propose that: 1) MGP and BGP genes originated from two genome duplications that occurred around 500 and 400 million years ago before jawless and jawed fish evolved, respectively; 2) MGP appeared first concomitantly with the emergence of cartilaginous structures, and BGP appeared thereafter along with bony structures; and 3) BGP derives from MGP. We also propose a highly specific pattern definition for the Gla domain of BGP and MGP. Previous Section Next Section BGP1 (bone Gla protein or osteocalcin) and MGP (matrix Gla protein) belong to the growing family of vitamin K-dependent (VKD) proteins, the members of which are involved in a broad range of biological functions such as skeletogenesis and bone maintenance (BGP and MGP), hemostasis (prothrombin, clotting factors VII, IX, and X, and proteins C, S, and Z), growth control (gas6), and potentially signal transduction (proline-rich Gla proteins 1 and 2). VKD proteins are characterized by the presence of several Gla residues resulting from the post-translational vitamin K-dependent γ-carboxylation of specific glutamates, through which they can bind to calcium-containing mineral such as hydroxyapatite. To date, VKD proteins have only been clearly identified in vertebrates (1) although the presence of a γ-glutamyl carboxylase has been reported in the fruit fly Drosophila melanogaster (2) and in marine snails belonging to the genus Conus (3). Gla residues have also been found in neuropeptides from Conus venoms (4), suggesting a wider prevalence of γ-carboxylation.
Peer review: yes
URI: http://hdl.handle.net/10400.1/4282
DOI: http://dx.doi.org/ 10.1074/jbc.M500257200
ISSN: 0021-9258
Versão do Editor: http://www.jbc.org/content/280/29/26659.long
Aparece nas colecções:CCM2-Artigos (em revistas ou actas indexadas)

FacebookTwitterDeliciousLinkedInDiggGoogle BookmarksMySpace
Formato BibTex MendeleyEndnote Degois 

Todos os registos no repositório estão protegidos por leis de copyright, com todos os direitos reservados.