Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.1/4298
Título: Existence of a tightly regulated water channel in saccharomyces cerevisiae
Autor: Meyrial, V.
Laizé, Vincent
Gobin, R.
Ripoche, P.
Hohmann, S.
Tacnet, F.
Palavras-chave: Aquaporin
Water transport
Saccharomyces cerevisiae
AQY1
AQY2
Data: Dez-2001
Editora: Wiley
Citação: Meyrial, V.; Laizé, V.; Gobin, R.; Ripoche, P.; Hohmann, S.; Tacnet, F.Existence of a tightly regulated water channel in saccharomyces cerevisiae, European Journal of Biochemistry, 268, 2, 334-343, 2001.
Resumo: The Saccharomyces cerevisiae strain Σ1278b possesses two putative aquaporins, Aqy1-1p and Aqy2-1p. Previous work demonstrated that Aqy1-1p functions as a water channel in Xenopus oocyte. However, no function could be attributed to Aqy2-1p in this system. Specific antibodies were used to follow the expression of Aqy1-1p and Aqy2-1p in the yeast. Aqy1-1p was never detected whatever the growth phase and culture conditions tested. In contrast, Aqy2-1p was detected only during the exponential growth phase in rich medium containing glucose. Aqy2-1p expression was repressed by hyper-osmotic culture conditions. Both immunocytochemistry and biochemical subcellular fractionation demonstrated that Aqy2-1p is located on the endoplasmic reticulum (ER) as well as on the plasma membrane. In microsomal vesicles enriched in ER, a water channel activity due to Aqy2-1p was detected by stopped-flow analysis. Our results show that the expression of aquaporins is tightly controlled. The physiological relevance of aquaporin-mediated water transport in yeast is discussed.
Peer review: yes
URI: http://hdl.handle.net/10400.1/4298
DOI: http://doi:10.1152/ 10.1046/j.1432-1033.2001.01882.x
ISSN: 0014-2956
Versão do Editor: http://onlinelibrary.wiley.com/doi/10.1046/j.1432-1033.2001.01882.x/abstract;jsessionid=E7A2EF635FAC2FF1164638504E16C526.f03t02
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