Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.1/4365
Título: Changes in the soluble bone proteome of reared white seabream (Diplodus sargus) with skeletal deformities
Autor: Silva, Tomé S.
Cordeiro, O.
Richard, N.
Conceição, L. E. C.
Rodrigues, Pedro
Palavras-chave: Aquaculture
Chemically assisted fragmentation
Differential expression
MASCOT
Skeletal deformities
Data: 2011
Editora: Elsevier
Citação: Silva, Tomé S.; Cordeiro, Odete; Richard, Nadège; Conceição, Luís E.C.; Rodrigues, Pedro M. Changes in the soluble bone proteome of reared white seabream (Diplodus sargus) with skeletal deformities, Comparative Biochemistry and Physiology Part D: Genomics and Proteomics, 6, 1, 82-91, 2011.
Resumo: One of the main constrains for commercial aquaculture production of white seabream (Diplodus sargus) is the high incidence of skeletal malformations in reared fish. The purpose of this study was to obtain a better understanding of the mechanisms involved in the development of these types of skeletal malformations by comparative proteomic analysis of the vertebral column of normal and deformed fish using 2DE for protein separation and MS for protein identification. We observed a 3.2 and 3.4-fold increase in the expression of two tropomyosin isoforms, one of which (tropomyosin-4) is essential for the motility and polarization cycles of osteoclasts. Furthermore, a 1.6, 1.7 and 1.8-fold increase in three parvalbumin spots was detected, suggesting a cellular response to increased intracellular Ca2+ levels. These results can be interpreted as signs of increased cellular activity in the bone of white seabream with skeletal deformities coupled to a higher degree of calcium mobilization, which elicits further studies into the use of these proteins as indicators of skeletal metabolic state.
Peer review: yes
URI: http://hdl.handle.net/10400.1/4365
DOI: http://dx.doi.org/10.1016/j.cbd.2010.03.008
ISSN: 1744-117X
Aparece nas colecções:CCM2-Artigos (em revistas ou actas indexadas)



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