Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.1/4471
Título: A comprehensive assessment of the transcriptome of cork oak (Quercus suber) through EST sequencing
Autor: Pereira-Leal, José B.
Abreu, Isabel A.
Alabaça, Cláudia S.
Almeida, Maria H.
Almeida, Paulo
Almeida, Tânia
Amorim, Maria I.
Araújo, Susana
Azevedo, Herlânder
Badia, Aleix
Batista, Dora
Bohn, Andreas
Capote, Tiago
Carrasquinho, Isabel
Chaves, Inês
Coelho, A. C.
Costa, Maria M. R.
Costa, Rita
Cravador, A.
Egas, Conceição
Faro, Carlos
Fortes, Ana M.
Fortunato, Ana S.
Gaspar, Maria J.
Gonçalves, Sónia
Graça, José
Horta, M.
Inácio, Vera
Leitão, J. M.
Lino-Neto, Teresa
Marum, Liliana
Matos, José
Mendonça, Diogo
Miguel, Andreia
Miguel, Célia M.
Morais-Cecílio, Leonor
Neves, Isabel
Nóbrega, Filomena
Oliveira, Maria M.
Oliveira, Rute
Pais, Maria S.
Paiva, Jorge A.
Paulo, O. S.
Pinheiro, Miguel
Raimundo, João A. P.
Ramalho, J. C.
Ribeiro, Ana I.
Ribeiro, Teresa
Rocheta, Margarida
Rodrigues, Ana I.
Rodrigues, José C.
Saibo, Nelson J. M.
Santo, Tatiana
Santos, Ana M.
Sá-Pereira, Paula
Sebastiana, Mónica
Simões, Fernanda
Sobral, Rómulo S.
Tavares, Rui
Teixeira, Rita
Varela, Carolina
Veloso, Maria M.
Ricardo, Cândido P. P.
Data: 15-Mai-2014
Editora: BioMed Central
Citação: BMC Genomics. 2014 May 15;15(1):371
Resumo: Background: Cork oak (Quercus suber) is one of the rare trees with the ability to produce cork, a material widely used to make wine bottle stoppers, flooring and insulation materials, among many other uses. The molecular mechanisms of cork formation are still poorly understood, in great part due to the difficulty in studying a species with a long life-cycle and for which there is scarce molecular/genomic information. Cork oak forests are of great ecological importance and represent a major economic and social resource in Southern Europe and Northern Africa. However, global warming is threatening the cork oak forests by imposing thermal, hydric and many types of novel biotic stresses. Despite the economic and social value of the Q. suber species, few genomic resources have been developed, useful for biotechnological applications and improved forest management. Results: We generated in excess of 7 million sequence reads, by pyrosequencing 21 normalized cDNA libraries derived from multiple Q. suber tissues and organs, developmental stages and physiological conditions. We deployed a stringent sequence processing and assembly pipeline that resulted in the identification of ~159,000 unigenes. These were annotated according to their similarity to known plant genes, to known Interpro domains, GO classes and E.C. numbers. The phylogenetic extent of this ESTs set was investigated, and we found that cork oak revealed a significant new gene space that is not covered by other model species or EST sequencing projects. The raw data, as well as the full annotated assembly, are now available to the community in a dedicated web portal at http://www.corkoakdb.org. Conclusions: This genomic resource represents the first trancriptome study in a cork producing species. It can be explored to develop new tools and approaches to understand stress responses and developmental processes in forest trees, as well as the molecular cascades underlying cork differentiation and disease response.
Peer review: yes
URI: http://hdl.handle.net/10400.1/4471
DOI: http://dx.doi.org/10.1186/1471-2164-15-371
Aparece nas colecções:FCT2-Artigos (em revistas ou actas indexadas)

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