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|Título:||Incubation temperature induces changes in muscle cellularity and gene expression in Senegalese sole (Solea senegalensis)|
Valente, L. M. P.
Conceição, L. E. C.
Fernandes, J. M. O.
|Citação:||Campos, C.; Valente, L.M.P.; Conceição, L.E.C.; Engrola, S.; Sousa, V.; Rocha, E.; Fernandes, J.M.O.Incubation temperature induces changes in muscle cellularity and gene expression in Senegalese sole (Solea senegalensis), Gene, 516, 2, 209-217, 2013.|
|Resumo:||Fertilised eggs of Senegalese sole were incubated at 15, 18 or 21 °C, and after hatching all larvae were reared at 21 °C until 30 days post-hatch. By this point larvae from the 18 or 21 °C temperature groups had 11 and 9% more muscle fibres than those from 15 °C, respectively. Hyperplastic growth during metamorphosis was higher in larvae from 18 °C. Embryonic temperature induced gene expression changes, albeit with a variable pattern throughout development. Myf5, myod2, myHC and fst mRNA levels were significantly higher at several stages prior to hatching in embryos incubated at 21 °C, whereas hsp90AB and hsp70 transcripts were present at higher levels in the 15 °C group. Myf5, myod1, myod2, pax7, myog, fst, igf-II, igf1r, hsp90AA and hsp90AB were expressed at higher levels during early development, particularly during somitogenesis. In contrast, mrf4, myHC, mylc2, igf-I, mstn1 and hsp70 were up-regulated at later stages of larval development, namely during and after metamorphosis. This study is the first example of thermal plasticity of myogenesis with prolonged effect in a flatfish.|
|Versão do Editor:||http://www.sciencedirect.com/science/article/pii/S0378111912016332|
|Aparece nas colecções:||CCM2-Artigos (em revistas ou actas indexadas)|
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|8. Incubation temperature induces changes in muscle cellularity and gene expression in Senegalese sole (Solea senegalensis).pdf||967,56 kB||Adobe PDF||Ver/Abrir Acesso Restrito. Solicitar cópia ao autor!|
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