Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.1/4628
Título: Incubation temperature induces changes in muscle cellularity and gene expression in Senegalese sole (Solea senegalensis)
Autor: Campos, C.
Valente, L. M. P.
Conceição, L. E. C.
Engrola, S.
Sousa, V.
Rocha, E.
Fernandes, J. M. O.
Palavras-chave: Solea senegalensis
Epigenetics
Development
Muscle cellularity
Thermal plasticity
Myogenesis
Data: 2013
Citação: Campos, C.; Valente, L.M.P.; Conceição, L.E.C.; Engrola, S.; Sousa, V.; Rocha, E.; Fernandes, J.M.O.Incubation temperature induces changes in muscle cellularity and gene expression in Senegalese sole (Solea senegalensis), Gene, 516, 2, 209-217, 2013.
Resumo: Fertilised eggs of Senegalese sole were incubated at 15, 18 or 21 °C, and after hatching all larvae were reared at 21 °C until 30 days post-hatch. By this point larvae from the 18 or 21 °C temperature groups had 11 and 9% more muscle fibres than those from 15 °C, respectively. Hyperplastic growth during metamorphosis was higher in larvae from 18 °C. Embryonic temperature induced gene expression changes, albeit with a variable pattern throughout development. Myf5, myod2, myHC and fst mRNA levels were significantly higher at several stages prior to hatching in embryos incubated at 21 °C, whereas hsp90AB and hsp70 transcripts were present at higher levels in the 15 °C group. Myf5, myod1, myod2, pax7, myog, fst, igf-II, igf1r, hsp90AA and hsp90AB were expressed at higher levels during early development, particularly during somitogenesis. In contrast, mrf4, myHC, mylc2, igf-I, mstn1 and hsp70 were up-regulated at later stages of larval development, namely during and after metamorphosis. This study is the first example of thermal plasticity of myogenesis with prolonged effect in a flatfish.
Peer review: yes
URI: http://hdl.handle.net/10400.1/4628
DOI: http://dx.doi.org/ 10.1016/j.gene.2012.12.074
ISSN: 1879-0038
Versão do Editor: http://www.sciencedirect.com/science/article/pii/S0378111912016332
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