Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.1/4803
Título: Use of focussed beam reflectance measurement (FBRM) for monitoring changes in biomass concentration
Autor: Whelan, Jessica
Murphy, Eilis
Pearson, Alan
Jeffers, Paul
Kieran, Patricia
McDonnell, Susan
Lima-Costa, Maria Emília
Glennon, Brian
Palavras-chave: Biomass concentration
On-line monitoring
FBRM
Cell morphology
Data: 2012
Editora: Springer
Citação: Whelan, Jessica; Murphy, Eilis; Pearson, Alan; Jeffers, Paul; Kieran, Patricia; McDonnell, Susan; Lima-Costa, Maria Emília; Glennon, Brian. Use of focussed beam reflectance measurement (FBRM) for monitoring changes in biomass concentration, Bioprocess and Biosystems Engineering, 35, 6, 963-975, 2012.
Resumo: The potential of focussed beam reflectance measurement (FBRM) as a tool to monitor changes in biomass concentration was investigated in a number of biological systems. The measurement technique was applied to two morphologically dissimilar plant cell suspension cultures, Morinda citrifolia and Centaurea calcitrapa, to a filamentous bacteria, Streptomyces natalensis, to high density cultures of Escherichia coli and to a murine Sp2/0 hybridoma suspension cell line, 3–2.19. In all cases, the biomass concentration proved to be correlated with total FBRM counts. The nature of the correlation varied between systems and was influenced by the concentration, nature, size and morphology of the particle under investigation.
Peer review: yes
URI: http://hdl.handle.net/10400.1/4803
DOI: http://dx.doi.org/ 10.1007/s00449-012-0681-9
ISSN: 1615-7591
Versão do Editor: http://link.springer.com/article/10.1007%2Fs00449-012-0681-9
Aparece nas colecções:CIM2-Artigos (em revistas ou actas indexadas)



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