Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.1/4836
Título: Characterization of Dunaliella salina strains by flow cytometry: a new approach to select carotenoid hyperproducing strains
Autor: Mendoza, Hector
De la Jara, Adelina
Freijanes, Karen
Carmona, Laura
Ramos, Ana
Duarte, Vanessa
Varela, J.
Palavras-chave: Flow cytometry
Carotenoids
Dunaliella salina
Microalgae
Data: 2008
Citação: Mendoza, H., K. Freijanes, L. Carmona, A. Ramos, V. Duarte, J. Varela & A. de la Jara. (2008). Characterization of Dunaliella salina strains by flow cytometry: a new technique to select β-carotene hyperproducing strains. Electronic Journal of Biotechnology, 11 (4): doi:10.2225/vol11-issue4-fulltext-2.
Resumo: In the present work the characterization of different strains of Dunaliella salina from established cell culture collections and various isolates from solar saltworks located in the Canary Island, as well as one mutant, was carried out in order to assess the existence of intraspecific differences and to determine the potential productivity of each one. Morphological characteristics such as cellular size and cellular complexity and parameters linked to cellular physiology, such as pigment content or cellular growth rate, were determined by means of traditional techniques as well as flow cytometry. Results showed a high morphological and physiological intraspecific variability among the studied strains. Results suggest that the application of the lipidic dye Nile red allowed the development of a cytometric method faster than the traditional techniques to select carotenoid hyperproducing strains of microalgae.
Peer review: yes
URI: http://hdl.handle.net/10400.1/4836
ISSN: 0717-3458
Versão do Editor: http://www.ejbiotechnology.info/index.php/ejbiotechnology/article/view/v11n4-2/637
Aparece nas colecções:FCT2-Artigos (em revistas ou actas indexadas)

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