Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.1/4838
Título: Isolation and characterization of a stress-inducible Dunaliella salina Lcy-β gene encoding a functional lycopene β-cyclase
Autor: Ramos, Ana
Coesel, Sacha
Marques, Ana
Rodrigues, Marta
Baumgartner, Alexandra
Noronha, João
Rauter, Amélia
Brenig, Bertram
Varela, J.
Palavras-chave: Carotenoid biosynthesis
Dunaliella salina
Lycopene β-cyclase
Gene expression
Stress response regulation
Data: 2008
Editora: Springer
Citação: Ramos, A., S. Coesel, A. Marques, M. Rodrigues, A. Baumgartner, J. Noronha, A. Rauter, B. Brenig & J. Varela (2008). Isolation and characterization of a stress-inducible Dunaliella salina Lcy-β gene encoding a functional lycopene beta-cyclase. Applied Microbiology and Biotechnology, 79: 819-828.
Resumo: The halotolerant green alga Dunaliella salina accumulates large amounts of β-carotene when exposed to various stress conditions. Although several studies concerning accumulation and biotechnological production of β-carotene have been published, the molecular basis and regulation of the genes involved in carotenoid biosynthesis in D. salina are still poorly known. In this paper, we report the isolation and regulation of the lycopene β-cyclase (Lcy-β) gene by abiotic stress. The function of this gene was determined by heterologous genetic complementation in E. coli. Gene expression and physiological analyses revealed that D. salina Lcy-β steady-state transcript and carotenoid levels were up-regulated in response to all stress conditions tested (salt, light and nutrient depletion). The results presented here suggest that nutrient availability is a key factor influencing carotenogenesis as well as carotenoid biosynthesis-related gene expression in D. salina.
Peer review: yes
URI: http://hdl.handle.net/10400.1/4838
DOI: http://dx.doi.org/10.1007/s00253-008-1492-4
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