Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.1/4857
Título: Efeitos in vivo de oligómeros de vanadato em marcadores de stresse oxidativo 
Autor: Gândara, Ricardo M. C.
Orientador: Aureliano, M.
Palavras-chave: Vanadato
Stress oxidativo
Data de Defesa: 2004
Resumo: Nowadays it is accepted that disturbances in transition metals homeostasis are related to changes in the oxidative status, depleting antioxidant substances and/or raising prooxidant ones or altering the activities of key enzymes in the antioxidant defence. Vanadium is probably involved in such effects. However when its toxicity is studied it is normally misregarded the formation of different vanadate oligomers with different structures, chemical properties and biochemical effects. Liver is one of the organs affected by transition-metals toxicity due to its accumulation. This work’s goal was to seek evidences that show different effects promoted by different vanadate oligomers. We have monitored several markers of oxidative stress by using fluorometric and spectroscopic techniques, and we analysed the in vivo effect of an acute exposure (12, 24 hours and 7 days), to a sub-lethal concentration (5 mM; 1 mg/kg) of two vanadium solutions (metavanadate and decavanadate), on hepatic tissue of Halobatrachus didactylus (toadfish). We observed that decavanadate promote different effects than metavanadate in several parameters, namely catalase activity, glutathione content, lipid peroxidation, mitochondrial superoxide anion production and vanadium accumulation. The results show that under defined experimental conditions, both solutions seem to equally depress ROS production as well as total intracellular reducing power.
Descrição: Relatório de estágio de licenciatura, Bioquímica, Faculdade de Ciências e Tecnologia. Universidade do Algarve, 2003
Peer review: yes
URI: http://hdl.handle.net/10400.1/4857
Aparece nas colecções:FCT1-Teses

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