Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.1/5386
Título: Cloning and expression of an elongation factor-1α in sea bream ( Sparus aurata ) larvae and adult tissue
Autor: Nowell, M. A.
Power, Deborah
Guerreiro, P. M.
Llewellyn, Lynda
Ramsurn, Vimi P.
Wigham, Trevor
Sweeney, Glen E.
Palavras-chave: EF-1a cDNA
Expression
Sea beam (sparus aurata)
Teleost
Larvae
Adult tissue
Data: Mar-2000
Editora: Springer
Citação: Mari A. Nowell, Deborah M. Power, Pedro M. Guerreiro, Lynda Llewellyn, Vimi Ramsurn, Trevor Wigham and Glen E. Sweeney. Cloning and Expression of an Elongation Factor-1a in Sea Bream (Sparus aurata) Larvae and Adult Tissue. Mar. Biotechnol. 2, 173–179, 2000
Resumo: A clone encoding the polypeptide elongation factor EF-1a was isolated from a complementary DNA library prepared from sea bream (Spartus aurata) larvae 1 to 10 days after hatching. The deduced amino acid sequence is between 82% and 95% similar to EF-1a in other animal species. EF-1a messenger RNA is present at low abundance in sea bream embryos prior to gastrulation, but at around 15 hours postfertilization, there is a 10-fold increase in transcript levels. This increase presumably reflects midblastula transition in this species. In adult sea bream, EF-1a appeared to have a relatively uniform distribution across all the tissues analyzed.
Peer review: yes
URI: http://hdl.handle.net/10400.1/5386
DOI: http://dx.doi.org/ 10.1007/s101269900022
ISSN: 1436-2228
Versão do Editor: http://link.springer.com/article/10.1007%2Fs101269900022
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