Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.1/5413
Título: Gene expression profiling of gilthead sea bream during early development and detection of stress-related genes by the application of cDNA microarray technology
Autor: Sarropoulou, Elena
Kotoulas, Georgios
Power, Deborah
Geisler, Robert
Palavras-chave: Sparus aurata
Stress response
Data: 2005
Editora: American Physiological Society
Citação: Elena Sarropoulou, Georgios Kotoulas, Deborah M. Power, Robert Geisler, "Gene expression profiling of gilthead sea bream during early development and detection of stress-related genes by the application of cDNA microarray technology" in Physiol Genomics. 2005 Oct 17;23(2):182-91.
Resumo: Gene expression profiling of gilthead sea bream during early development and detection of stress-related genes by the application of cDNA microarray technology. Physiol Genomics 23: 182–191, 2005. First published July 26, 2005; doi:10.1152/physiolgenomics.00139.2005.—Large-scale gene expression studies were performed for one of the main European aquaculture species, the gilthead sea bream Sparus auratus L. For this purpose, a cDNA microarray containing 10,176 clones from a cDNA library of mixed embryonic and larval stages was constructed. In addition to its importance for aquaculture, the taxonomic position and the relatively small genome size of sea bream makes it a prospective model for evolutionary biology and comparative genomics. However, so far, no large-scale analysis of gene expression exists for this species. In the present study, gene expression was analyzed in gilthead sea bream during early development, a significant period in the determination of quantitative traits and therefore of considerable interest for aquaculture. Synexpression groups expressed primarily early and late in development were determined and were composed of both known and novel genes. Furthermore, it was possible to identify stress response genes induced by cortisol injections using the cDNA microarray generated. The creation of gene expression profiles for sea bream by microarray hybridization will accelerate identification of candidate genes involved in multifactorial traits and certain regulatory pathways and will also contribute to a better understanding of the genetic background of fish physiology, which may help to improve aquaculture practices.
Peer review: yes
URI: http://hdl.handle.net/10400.1/5413
DOI: http://dx.doi.org/10.1152/physiolgenomics.00139.2005
ISSN: 1094-8341
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