Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.1/6153
Título: Porcine D-amino acid oxidase: determination of the mRNA nucleotide sequence by the characterization of genomic and cDNA clones
Autor: Jacobs, P.
Brockly, F.
Massaer, M.
Loriau, R.
Guillaume, J. P.
Ciccarelli, E.
Heinderyckx, M.
Cravador, A.
Biemans, R.
Van Elsen, A.
Herzog, A
Bollen, A.
Data: 1987
Resumo: Oligodeoxynucleotide probes derived from the published amino acid (aa) sequence for D-amino acid oxidase (DAO) [Ronchi et al. J. Biol. Chem. 259 (1982) 8824-8834] were used to screen cDNA libraries made from porcine kidney cortex and liver. Whereas no clones were obtained from kidney mRNAs, 20 independent ones were isolated from the liver library. Surprisingly, all of them carried only partial cDNAs for DAO starting around aa 100 in the coding sequence and extending for up to 250 bp in the 3'-noncoding sequence. One of these clones, pULB9103, was used to screen a porcine genomic library and allowed the isolation of DAO gene clone phULB001. Four exons encoding aa 1-151 were identified and sequenced, as well as the relevant exon-intron junctions. The mRNA sequence coding for DAO has been reconstituted from the genomic and cDNA sequences; its analysis by computer did not reveal and significant secondary structure, or particular feature, which could explain the failure to obtain full-length cDNAs.
Peer review: yes
URI: http://hdl.handle.net/10400.1/6153
ISSN: 0378-1119
Aparece nas colecções:FCT2-Artigos (em revistas ou actas indexadas)

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