Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.1/6212
Título: Variability of a portion of RNA 3 containing the coat protein gene of Citrus variegation virus (CVV) using single-strand conformation polymorphism (SSCP)
Autor: Bennani, B.
Zemzami, M.
Nolasco, Gustavo
Azeddoug, H.
Data: 2002
Resumo: A portion of RNA 3 of Citrus variegation virus (CVV), comprising part of the intergenic region and the coat protein (CP) gene from eight viral isolates, was amplified by RT-PCR and cloned. The clones were compared for intra and inter-isolate variations by single-strand conformation polymorphism analysis. Some of the results were compared with sequence data previously obtained. The test discriminated between clones differing in as little as 3.2% of the nucleotides. Most isolates included several variants, in some cases with a predominant pattern, which, however, could no longer be recognised in new RT-PCR products obtained 13 months later. This procedure can there-fore be used to identify and detect variations between CVV isolates. It is rapid, inexpensive and may reduce the amount of sequencing needed for comparing viral isolates.
Peer review: yes
URI: http://hdl.handle.net/10400.1/6212
ISSN: 0031-9465
Aparece nas colecções:FCT2-Artigos (em revistas ou actas indexadas)

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