Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.1/6230
Título: The p19.7 RNA silencing suppressor from Grapevine leafroll-associated virus 3 shows different levels of activity across phylogenetic groups
Autor: Gouveia, P.
Nolasco, Gustavo
Data: 2012
Editora: Springer Verlag
Resumo: At least five phylogenetic groups have been reported for Grapevine leafroll-associated virus 3 (GLRaV-3). The p19.7 protein encoded by the GLRaV-3 was previously identified as an RNA silencing suppressor. In this study, five constructs of p19.7 belonging to different groups were compared for their suppressing activity. For each p19.7 variant, the accumulation level of green fluorescent protein mRNA and specific siRNAs were determined using co-infiltration assays in transgenic 16C Nicotiana benthamiana. Differences in the suppressing activity were found among the variants assayed. Some constructs originated viral-like mosaic symptoms that evolved to necrosis. The intensity of these symptoms appeared to be related to the strength of the suppressor activity. A comparison of the protein sequences revealed a few amino acid substitutions that may be associated with the observed differences in the suppressing activity.
Peer review: yes
URI: http://hdl.handle.net/10400.1/6230
DOI: https://dx.doi.org/10.1007/s11262-012-0772-3
ISSN: 0920-8569
Aparece nas colecções:FCT2-Artigos (em revistas ou actas indexadas)

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