Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.1/6427
Título: Cryopreservation of shoot tips from the endangered endemic species Tuberaria major
Autor: Coelho, Natacha
González-Benito, María Elena
Romano, Anabela
Palavras-chave: Vitrification
Encapsulation-dehydration
Ex situ conservation
Long-term conservation
Data: 2014
Editora: Springer Verlag
Resumo: Tuberaria major is an endangered endemic species from the Algarve, in the south of Portugal. We investigated two techniques for the cryopreservation of T. major shoot tips, namely vitrification and encapsulation-dehydration. Before the cryopreservation trials, shoot tips were precultured for 1 day on liquid Murashige and Skoog (MS) medium containing 0.3 M sucrose. For the vitrification method, shoots tips were exposed for 0, 30, 60, 90 and 120 min to plant vitrification solution 2 (PVS2). As for the encapsulation-dehydration method, shoot tips were dried inside a laminar air flow cabinet for 0, 1, 2, 3, 4, 5 and 6 h at room temperature. The highest regrowth percentages were approximately 60 and 67 % for vitrification and encapsulation-dehydration, respectively. The best times were 60 min exposure to PVS2 for vitrification and 3 h desiccation for encapsulation-dehydration. Though these are preliminary results, the use of the cryopreservation techniques tested here proved to be an important asset in the conservation of this endangered species and will complement the conservation strategies previously developed.
Peer review: yes
URI: http://hdl.handle.net/10400.1/6427
DOI: http://dx.doi.org/10.1007/s11738-014-1678-6
ISSN: 0137-5881
Aparece nas colecções:FCT2-Artigos (em revistas ou actas indexadas)



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