Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.1/6430
Título: In vitro morphogenesis in zygotic embryo cultures of carob tree (Ceratonia siliqua L.)
Autor: Custódio, Luísa
Romano, Anabela
Palavras-chave: Conversion
Regeneration
Organogenesis
Somatic embryogenesis
Tissue culture
Data: 2006
Editora: International Society for Horticultural Science
Resumo: In this study, organogenic cultures were induced from zygotic embryos of carob tree. Basal medium consisted of Murashige & Tucker (1969), supplemented with 0.5 or 1 mg L-1 of 2,4-dichlorophenoxyacetic acid alone or combined with 0.5, 1, 2, 3 and 4 mg L-1 of kinetin. Calli initiation occurred in all the hormonal combinations tested. The zygotic embryo cultures differentiated two types of regenerants: somatic embryos (SEs) and shoots, with a maximum frequency of 30 and 100%, respectively. SEs exhibited morphological abnormalities, such as fusion and absence of cotyledons, and showed secondary embryogenesis. The regenerated shoots were rooted, hardened and transferred to the soil.
Peer review: yes
URI: http://hdl.handle.net/10400.1/6430
DOI: http://dx.doi.org/10.17660/ActaHortic.2006.725.68
ISBN: 978-90-6605-719-7
ISSN: 0567-7572
Aparece nas colecções:FCT2-Artigos (em revistas ou actas indexadas)



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