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- 2.P3. Cloning and gene expression of two novel stanniocalcin genes in the puffer fish Tetraodon nigroviridisPublication . Schein, V; Schein, Vanessa; Pinto, Patricia IS; Canario, Adelino V. M.Stanniocalcin 1 (STC1) is a hypocalcemic hormone first described in fishes. Recently a second gene (STC2) was isolated from mammals and fishes. However, in silico analysis of Tetraodon nigroviridis and Takifugu rubripes genomes revealed the presence of a third and a fourth gene. In this study STC1 was cloned from de Corpuscles of Stannius (CS), STC2 from the muscle, and the two novel genes identified, STC1B and STC2B from the brain of Tetraodon. The STC-1B gene encodes an ORF of 287aa and contains 11 conserved cyteine residues and the Nlinked glycosylation site, present in all identified STC1.
- Immunohistochemistry of stanniocalcin isoforms in fish tissuesPublication . Schein, V; Schein, Vanessa; Silva, Nádia; Power, Deborah; Canario, Adelino V. M.Stanniocalcin 1 (STC1) is a homodimeric glycoprotein synthesized and secreted by the Corpuscles of Stannius in response to a rise in serum Ca2+. Although STC1 has received most attention, at least one other isoform, STC2, encoded by a separate gene has been identified in all vertebrates. Taking into consideration the duplication events in teleost genomes we decided to investigate the presence of STC paralogues in teleost fish genomes.