Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.1/10913
Título: The use of different anaesthetics as welfare promoters during short-term human manipulation of European cuttlefish (Sepia officinalis) juveniles
Autor: Gonçalves, Rui A.
Aragão, Cláudia
Frias, Paulo A.
Sykes, António V.
Palavras-chave: 2-Phenoxyethanol
AQUI-S®
Clove oil
Ethanol hypothermia
Magnesium chloride
MS-222®
Data: 2012
Editora: Elsevier
Resumo: Anaesthetics are frequently used to prevent injuries, stress and to promote welfare while handling animals. The efficiency of five anaesthetics for short-term handling of the European cuttlefish (Sepia officinalis) was tested in order to determine the best agent and its concentration. Ethanol (10.0, 20.0 and 30.0 mL.L−1), clove oil (0.05, 0.15 mL.L−1), hypothermia (8 °C), magnesium chloride (hexahydrated; 20.0, 27.0 g.L−1) and AQUI-S® (10.9 μl.L−1) were the selected anaesthetics. This choice was preceded by a preliminary trial, with additional agents, where 2-phenoxyethanol (10, 15, 20, 30 mL.L−1) and MS-222® (30, 40 mg.L−1) were used. Due to the lack of reliable results from these two agents, they were discarded. Anaesthetic procedure was performed in 85 cuttlefish juveniles with a mean wet weight of 45.69±12.01 g, on a 5 L container, under hyper-oxygenated seawater (~200%), plus the dose of a given anaesthetic agent. After achieving induction, cuttlefish were handled for 180 s, which consisted in a weighing procedure according to a developed CCMAR protocol. Recovery from anaesthesia was performed in a tank with flow-through hyper-oxygenated water. All these procedures were video recorded and used to, a posteriori, obtain the time frames of induction, handling and recovery, as well as for the behaviour analysis during each stage.
Peer review: yes
URI: http://hdl.handle.net/10400.1/10913
DOI: 10.1016/j.aquaculture.2012.10.014
ISSN: 0044-8486
Aparece nas colecções:CCM2-Artigos (em revistas ou actas indexadas)

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