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|Título:||The use of different anaesthetics as welfare promoters during short-term human manipulation of European cuttlefish (Sepia officinalis) juveniles|
|Autor:||Gonçalves, Rui A.|
Frias, Paulo A.
Sykes, António V.
|Resumo:||Anaesthetics are frequently used to prevent injuries, stress and to promote welfare while handling animals. The efficiency of five anaesthetics for short-term handling of the European cuttlefish (Sepia officinalis) was tested in order to determine the best agent and its concentration. Ethanol (10.0, 20.0 and 30.0 mL.L−1), clove oil (0.05, 0.15 mL.L−1), hypothermia (8 °C), magnesium chloride (hexahydrated; 20.0, 27.0 g.L−1) and AQUI-S® (10.9 μl.L−1) were the selected anaesthetics. This choice was preceded by a preliminary trial, with additional agents, where 2-phenoxyethanol (10, 15, 20, 30 mL.L−1) and MS-222® (30, 40 mg.L−1) were used. Due to the lack of reliable results from these two agents, they were discarded. Anaesthetic procedure was performed in 85 cuttlefish juveniles with a mean wet weight of 45.69±12.01 g, on a 5 L container, under hyper-oxygenated seawater (~200%), plus the dose of a given anaesthetic agent. After achieving induction, cuttlefish were handled for 180 s, which consisted in a weighing procedure according to a developed CCMAR protocol. Recovery from anaesthesia was performed in a tank with flow-through hyper-oxygenated water. All these procedures were video recorded and used to, a posteriori, obtain the time frames of induction, handling and recovery, as well as for the behaviour analysis during each stage.|
|Aparece nas colecções:||CCM2-Artigos (em revistas ou actas indexadas)|
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|Gonçalves et al. 2012.pdf||341,93 kB||Adobe PDF||Ver/Abrir Acesso Restrito. Solicitar cópia ao autor!|
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