Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.1/1322
Título: Effect of harvesting stress and storage conditions on protein degradation in fillets of farmed gilthead seabream (Sparus aurata): a differential scanning calorimetry study
Autor: Matos, Elisabete
Silva, Tomé S.
Tiago, Teresa
Aureliano, M.
Dinis, Maria Teresa
Dias, J.
Palavras-chave: DSC
Fish storage
Data: 2011
Editora: Elsevier
Resumo: A trial was undertaken to evaluate Differential Scanning Calorimetry (DSC) as a fast analytical tool to differentiate gilthead seabream subjected to variable conditions of slaughter stress and post-mortem storage. Fish were subjected to different harvesting stress conditions: profound anaesthesia (PA, low stress) and net crowding (NC, high stress). Fish were slaughtered in an ice-salt water slurry, and subsequently stored on ice (7 days). Additional NC fish were frozen ( 20 C) and subjected to a freeze–thaw cycle. Dorsal muscle was assessed for cathepsins activity, liquid loss and DSC analysis. It is demonstrated that DSC analysis is capable of differentiating fresh, frozen and thawed-re-frozen fish, while liquid loss and cathepsin B activity are good markers to distinguish fresh from frozen fish. Harvesting stress had little effect on myosin and actin enthalpy transitions, as observed by DSC at 49 and 74 C, respectively, but a lower DH actin/myosin ratio was found in PA fish, suggesting that intense exercise prior to slaughter promoted partial denaturation of muscle myosin.
Peer review: yes
URI: http://hdl.handle.net/10400.1/1322
ISSN: 0308-8146
Aparece nas colecções:FCT2-Artigos (em revistas ou actas indexadas)

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