Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.1/2557
Título: In vitro minimum growth for conservation of Drosophyllum lusitanicum
Autor: Gonçalves, Sandra
Romano, Anabela
Palavras-chave: Endangered species
Germplasm conservation
Osmotic stress
Data: 2007
Citação: Gonçalves S., Romano A. 2007. In vitro minimal growth conservation of Drosophyllum lusitanicum (L.) Link, Biologia Plantarum 51: 795-798
Resumo: The present paper reports a protocol for minimum growth conservation of Drosophyllum lusitanicum (L.) Link. in vitro. Double-node cuttings were maintained for 4, 8 and 12 months at 5 or 25 ºC in the dark. The effects of sucrose either alone at 5, 20, 30, 40 and 60 g dm-3 or at 20, 40 and 60 g dm-3 in combination with 20 g dm-3 mannitol, on survival and post-storage shoot multiplication efficiency were investigated. The cultures could effectively be conserved under minimum growth at 5 ºC for 8 months on Murashige and Skoog’s medium supplemented with 60 g dm-3 sucrose, 20 g dm-3 mannitol and 0.91 μM zeatin. Following extended conservation, the cultures could be successfully regenerated into new shoots, and they were morphologically similar to those of non-stored controls.
Peer review: yes
URI: http://hdl.handle.net/10400.1/2557
ISSN: 0006-3134
Versão do Editor: http://link.springer.com/article/10.1007%2Fs10535-007-0163-0?LI=true
Aparece nas colecções:FCT2-Artigos (em revistas ou actas indexadas)

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