Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.1/2559
Título: An efficient in vitro propagation protocol for Pinguicula lusitanica, a rare insectivorous plant
Autor: Gonçalves, Sandra
Escapa, Ana Luísa
Grevenstuk, Tomás
Romano, Anabela
Palavras-chave: Conservation
In vitro regeneration
Plant growth regulators
Plantlet production
Data: 2008
Citação: Gonçalves S., Escapa A. L., Grevenstuk T., Romano A. 2008. An efficient in vitro propagation protocol for Pinguicula lusitanica, a rare insectivorous plant, Plant Cell, Tissue and Organ Culture 95: 239-243
Resumo: In this study, an efficient protocol was developed for in vitro propagation of Pinguicula lusitanica L., a rare insectivorous plant with pharmacological value and limited reproductive capacity. The effects of two concentrations (0.1 and 0.5 mg l-1) of a range of plant growth regulators, including cytokinins (BA, KIN, and ZEA) and auxins (IAA, IBA, and NAA), and three concentrations of MS medium macronutrients (total, 1/2 and 1/4MS) on proliferation and rooting, were investigated. P. lusitanica shoots showed abundant proliferation and rooting capacity, both of which were significantly influenced by MS medium strength. The use of 1/2MS supplemented with 0.5 mg l-1 BA or KIN ensure a 29-fold rate of proliferation. Best rooting frequency and higher root number and length were attained in 1/4MS medium containing 0.2 mg l-1 IAA. Sixty percent of the plantlets were successfully acclimatized to ex vitro conditions, exhibiting normal development. These plantlets can be used to replenish declining populations in the wild and/or for the extraction of bioactive compounds, reducing pressure on wild stocks.
Peer review: yes
URI: http://hdl.handle.net/10400.1/2559
ISSN: 0167-6857
Versão do Editor: http://link.springer.com/article/10.1007%2Fs11240-008-9423-8
Aparece nas colecções:FCT2-Artigos (em revistas ou actas indexadas)

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