Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.1/2698
Título: Transthoracic echocardiography reference values in juvenile and adult 129/Sv mice
Autor: Vinhas, Maurícia
Araújo, Ana Carolina
Ribeiro, Sónia
Rosário, Luís Brás
Belo, José
Data: 1-Mai-2013
Editora: BioMed Central
Citação: Cardiovascular Ultrasound. 2013 May 01;11(1):12
Resumo: Background In the recent years, the use of Doppler-echocardiography has become a standard non-invasive technique in the analysis of cardiac malformations in genetically modified mice. Therefore, normal values have to be established for the most commonly used inbred strains in whose genetic background those mutations are generated. Here we provide reference values for transthoracic echocardiography measurements in juvenile (3 weeks) and adult (8 weeks) 129/Sv mice. Methods Echocardiographic measurements were performed using B-mode, M-mode and Doppler-mode in 15 juvenile (3 weeks) and 15 adult (8 weeks) mice, during isoflurane anesthesia. M-mode measurements variability of left ventricle (LV) was determined. Results Several echocardiographic measurements significantly differ between juvenile and adult mice. Most of these measurements are related with cardiac dimensions. All B-mode measurements were different between juveniles and adults (higher in the adults), except for fractional area change (FAC). Ejection fraction (EF) and fractional shortening (FS), calculated from M-mode parameters, do not differ between juvenile and adult mice. Stroke volume (SV) and cardiac output (CO) were significantly different between juvenile and adult mice. SV was 31.93 ± 8.67 μl in juveniles vs 70.61 ± 24.66 μl in adults, ρ < 0.001. CO was 12.06 ± 4.05 ml/min in juveniles vs 29.71 ± 10.13 ml/min in adults, ρ < 0.001. No difference was found in mitral valve (MV) and tricuspid valve (TV) related parameters between juvenile and adult mice. It was demonstrated that variability of M-mode measurements of LV is minimal. Conclusions This study suggests that differences in cardiac dimensions, as wells as in pulmonary and aorta outflow parameters, were found between juvenile and adult mice. However, mitral and tricuspid inflow parameters seem to be similar between 3 weeks and 8 weeks mice. The reference values established in this study would contribute as a basis to future studies in post-natal cardiovascular development and diagnosing cardiovascular disorders in genetically modified mouse mutant lines.
Peer review: yes
URI: http://hdl.handle.net/10400.1/2698
DOI: http://dx.doi.org/10.1186/1476-7120-11-12
Aparece nas colecções:FCT2-Artigos (em revistas ou actas indexadas)

Ficheiros deste registo:
Ficheiro Descrição TamanhoFormato 
1476-7120-11-12.xml108,14 kBXMLVer/Abrir
1476-7120-11-12.pdf1,32 MBAdobe PDFVer/Abrir
1476-7120-11-12-S3.DOCX19,41 kBMicrosoft Word XMLVer/Abrir
1476-7120-11-12-S2.DOCX19,56 kBMicrosoft Word XMLVer/Abrir
1476-7120-11-12-S1.DOCX13,14 kBMicrosoft Word XMLVer/Abrir


FacebookTwitterDeliciousLinkedInDiggGoogle BookmarksMySpace
Formato BibTex MendeleyEndnote 

Todos os registos no repositório estão protegidos por leis de copyright, com todos os direitos reservados.