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|Título:||Five gonadotrophin-releasing hormone receptors in a teleost fish: isolation, tissue distribution and phylogenetic relationships|
|Autor:||Moncaut, Natalia P.|
Somoza, G. M.
Canario, Adelino V. M.
|Editora:||Society for Endocrinology|
|Citação:||Moncaut, N.; Somoza, G. M.; Power, D. M.; Canario, A. V. M.Five gonadotrophin-releasing hormone receptors in a teleost fish: isolation, tissue distribution and phylogenetic relationships, Journal of Molecular Endocrinology, 34, 3, 767-779, 2005|
|Resumo:||Gonadotrophin-releasing hormone (GnRH) is the main neurohormone controlling gonadotrophin release in all vertebrates, and in teleost ﬁsh also of growth hormone and possibly of other adenohypophyseal hormones. Over 20 GnRHs have been identiﬁed in vertebrates and protochoordates and shown to bind cognate G-protein couple receptors (GnRHR). We have searched the puffer ﬁsh, Fugu rubripes, genome sequencing database, identiﬁed ﬁve GnRHR genes and proceeded to isolate the corresponding complementary DNAs in European sea bass, Dicentrachus labrax. Phylogenetic analysis clusters the European sea bass, puffer ﬁsh and all other vertebrate receptors into two main lineages corresponding to the mammalian type I and II receptors. The ﬁsh receptors could be subdivided in two GnRHR1 (A and B) and three GnRHR2 (A, B and C) subtypes. Amino acid sequence identity within receptor subtypes varies between 70 and 90% but only 50–55% among the two main lineages in ﬁsh. All European sea bass receptor mRNAs are expressed in the anterior and mid brain, and all but one are expressed in the pituitary gland. There is differential expression of the receptors in peripheral tissues related to reproduction (gonads), chemical senses (eye and olfactory epithelium) and osmoregulation (kidney and gill). This is the ﬁrst report showing ﬁve GnRH receptors in a vertebrate species and the gene expression patterns support the concept that GnRH and GnRHRs play highly diverse functional roles in the regulation of cellular functions, besides the ‘‘classical’’ role of pituitary function regulation.|
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