Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.1/4285
Título: Alternatively spliced transcripts of Sparus aurata insulin-like growth factor 1 are differentially expressed in adult tissues and during early development
Autor: Tiago, Daniel M.
Laizé, Vincent
Cancela, Leonor
Palavras-chave: Pro-insulin-like growth factor 1 (proIGF-1)
Alternative splicing
E domain
Gilthead seabream (Sparus aurata)
Data: Jun-2008
Editora: Elsevier
Citação: Tiago DM; Laizé V; Cancela ML. Alternatively spliced transcripts of Sparus aurata insulin-like growth factor 1 are differentially expressed in adult tissues and during early development, General and Comparative Endocrinology, 157, 2, 107-115, 2008.
Resumo: Spliced variants of insulin-like growth factor 1 (IGF-1), a small peptide with a critical role in metabolism and growth, have been identified in various vertebrate species. However, despite recent functional data in mammalian systems suggesting specific roles (e.g. in muscle formation) for their pro-peptides and/or E domains, their function remains unclear. In this study, three alternatively spliced variants of Sparus aurata proIGF-1 (1a, 1b, and 1c) were identified and their expression analyzed. In adult fish, IGF-1 gene expression was observed in various soft tissues (highest levels in liver) and calcified tissues, with IGF-1c being always the most expressed isoform. In developing larvae, each isoform presented a specific pattern of expression, characterized by different onset and extent and consistent with a possible role of IGF-1a and 1b during early post-hatching events (e.g. bone or muscle formation), while IGF-1c would be rather involved in early larvae formation but probably acts in concerted action with other isoforms at later stages. We also propose that, in adults, IGF-1a and 1b isoforms may have a local action, while isoform 1c would assume a systemic action, as its mammalian counterpart. This hypothesis was further supported by in silico analysis of isoform distribution, revealing that only IGF-1c/Ea isoform has been conserved throughout evolution and that other fish isoforms (i.e. 1a and 1b) may be associated with mechanisms of osmoregulation. We finally propose that IGF-1 variants may exhibit different modes of action (systemic or local) and may be involved in different developmental and adaptive mechanisms.
Peer review: yes
URI: http://hdl.handle.net/10400.1/4285
DOI: http://dx.doi.org/ 10.1016/j.ygcen.2008.04.006
ISSN: 0016-6480
Versão do Editor: http://www.sciencedirect.com/science/article/pii/S0016648008001603
Aparece nas colecções:CCM2-Artigos (em revistas ou actas indexadas)



FacebookTwitterDeliciousLinkedInDiggGoogle BookmarksMySpace
Formato BibTex MendeleyEndnote 

Todos os registos no repositório estão protegidos por leis de copyright, com todos os direitos reservados.