Gavaia, Paulo J.Simes, DOrtiz-Delgado, J. B.Sarasquete, C.Cancela, Leonor2014-06-252014-06-25200311. Gavaia P.J, Simes D.C., Ortiz J.B., Sarasquete M.C., Cancela M.L. 2003. Simultaneous detection of osteocalcin and matrix gla protein in developmental stages of zebra fish (Danio rerio). 30th European Symposium on Calcified Tissues, Palazzo dei Congressi. May 8-12, Rome, Italy.AUT: LCA00739; AUT: DSI00813;http://hdl.handle.net/10400.1/4495Osteocalcin (Bone Gla Protein, BGP) and Matrix Gla Protein (MGP) are gammacarboxylated calcium-binding proteins that have been only recently identified in fish. We have previously purified and characterized the two proteins from zebra fish calcified tissues. Polyclonal antibodies against Argyrosomus regius BGP and MGP were validated by western blot assay. The immunolocalization of BGP and MGP in zebra fish was performed in plastic sections of larvae and juveniles, covering all major developmental stages.engconference object2014-05-30http://dx.doi.org/10.1007/s00223-003-5002-5