Rodrigues, PedroGraça, FernandoMacedo, Anjos L.Moura, IsabelMoura, José J. G.2014-06-182014-06-182001Rodrigues, Pedro; Graça, Fernando; Macedo, Anjos L.; Moura, Isabel; Moura, José J.G. Characterization of Recombinant Desulfovibrio gigas Ferredoxin, Biochemical and Biophysical Research Communications, 289, 2, 630-633, 2001.0006-291XAUT: PMR01268;http://hdl.handle.net/10400.1/4361Dg ferredoxin gene was cloned using the polymerase chain reaction (PCR), inserted into vector pT7-7, and overexpressed in Escherichia coli (E. coli) grown in aerobic media. The recombinant protein is a dimer and contains a [3Fe–4S] cluster per monomer. EPR and 1H NMR data of recombinant and wild-type protein are compared.engFerredoxin3Fe–4S clusterIron–sulfur clusterParamagnetic proteinFerredoxin genejournal article2014-06-12http://dx.doi.org/10.1006/bbrc.2001.6016