Pacchiarini, T.Cabrita, ElsaCross, I.Ortiz-Delgado, J. B.Leite, RicardoGavaia, Paulo J.Herráez, M. P.Rebordinos, L.Sarasquete, C.2014-06-182014-06-182011T. Pacchiarini, E. Cabrita, I. Cross, J.B. Ortiz-Delgado, R. Leite, P.J. Gavaia, M.P. Herráez, L. Rebordinos, C. Sarasquete 2011. Two isoforms of vasa maternal factor in Senegalese sole: Biotechnological applications. Workshop - The cultivation of Soles V. 5-7 April 2011, CCMAR, University of Algarve, Faro, Portugal.http://hdl.handle.net/10400.1/4371Primordial Germ Cells (PGCs) identification and manipulation present considerable potential for hatchery practice and surrogate broodstocks. To carry out the PGCs characterization a specific molecular marker is required. The vasa gene is a good candidate to identify PGCs and others germinal cells (Nagasawa et al., 2009). The aim of this study was the cloning of the Solea senegalensis vasa cDNA and its expression pattern during early development and adulthood.engother