Pereira, HugoBarreira, LuísaMozes, AndreFlorindo, C.Polo, CristinaDuarte, Catarina V.Custódio, LuísaVarela, J.2012-02-292012-02-292011-12-22Biotechnology for Biofuels. 2011 Dec 22;4(1):61AUT: LBA00541; JVA01305;http://hdl.handle.net/10400.1/917Abstract We describe a new selection method based on BODIPY (4,4-difluoro-1,3,5,7-tetramethyl-4-bora-3a,4a-diaza-s-indacene) staining, fluorescence activated cell sorting (FACS) and microplate-based isolation of lipid-rich microalgae from an environmental sample. Our results show that direct sorting onto solid medium upon FACS can save about 3 weeks during the scale-up process as compared with the growth of the same cultures in liquid medium. This approach enabled us to isolate a biodiverse collection of several axenic and unialgal cultures of different phyla.engjournal article2012-02-27Pereira et al.; licensee BioMed Central Ltd.http://dx.doi.org/10.1186/1754-6834-4-61