Oliveira, DanielSilva, Tome S.Cordeiro, Odete D.Cavaco, Sofia I.Simes, Dina2018-12-072018-12-0720121537-744Xhttp://hdl.handle.net/10400.1/12065Nacre, when implanted in vivo in bones of dogs, sheep, mice, and humans, induces a biological response that includes integration and osteogenic activity on the host tissue that seems to be activated by a set of proteins present in the nacre water-soluble matrix (WSM). We describe here an experimental approach that can accurately identify the proteins present in the WSM of shell mollusk nacre. Four proteins (three gigasin-2 isoforms and a cystatin A2) were for the first time identified in WSM of Crassostrea gigas nacre using 2DE and LC-MS/MS for protein identification. These proteins are thought to be involved in bone remodeling processes and could be responsible for the biocompatibility shown between bone and nacre grafts. These results represent a contribution to the study of shell biomineralization process and opens new perspectives for the development of new nacre biomaterials for orthopedic applications.engOyster pinctada-fucataTenascin-COrganic matrixCathepsin-KCysteine proteinasesBone-resorptionCystatin-CIn-VivoPacific oysterStefin-Ajournal article10.1100/2012/765909