Laizé, VincentRousselet, G.Verbavatz, J. M.Berthonaud, V.Gobin, R.Roudier, N.Abrami, L.Ripoche, P.Tacnet, F.2014-06-062014-06-061995-10-16Laizé V; Rousselet G; Verbavatz JM; Berthonaud V; Gobin R; Roudier N; Abrami L; Ripoche P; Tacnet F. Functional expression of the human CHIP28 water channel in a yeast secretory mutant, FEBS Letters, 373, 3, 269-274, 1995.0014-5793http://hdl.handle.net/10400.1/4216The temperature-sensitive Saccharomyces cerevisiae mutant strain NY17, deficient in the secretory pathway (sec6-4 mutation), is used for the heterologous expression of the human CHIP28 water channel. After a heat-shock, the protein is present in partially purified post-golgi secretory vesicles, lmmunodetection and water transport studies, directly made on the vesicles, showed that CHIP28 is highly expressed and active in the yeast membranes.engSaccharomyces cerevisiae sec6-4 nutantPost-golgi secretory vesiclesWater transportAQP1journal article2014-06-03http://dx.doi.org/10.1016/0014-5793(95)01060-R