Solid phase non isotopic labelling of oligodeoxynucleotides using 5'-protected aminoalkyl phosphoramidites: Application to the specific detection of human papilloma virus DNA

De Vos, M.J.Cravador, A.Lenders, J. P.Houard, S.Bollen, A.2015-06-152015-06-1519900732-8311AUT: ACR00659;http://hdl.handle.net/10400.1/6139Phosphoramidites of thymidine or 2'-deoxyinosine, modified in 5' by the addition of an aminoalkylcarbamate function, were prepared. The derivatized nucleotides can be used in automatic DNA synthesis to tag any oligodeoxynucleotide chain and provide a convenient reactive group for labelling with non radioactive reporters. As an example of application, we show the specific detection of Human Papilloma Virus DNA using a biotin-labelled 29-mer oligodeoxynucleotide entirely prepared on solid support.engSolid phase non isotopic labelling of oligodeoxynucleotides using 5'-protected aminoalkyl phosphoramidites: Application to the specific detection of human papilloma virus DNAjournal article