Rodrigues, Maria JoãoOliveira, M.Neves, V.Ovelheiro, AndreiaPereira, C. A.Neng, N. R.Nogueira, J. M. F.Varela, J.Barreira, LuísaCustódio, Luísa2020-07-242020-07-242019-010254-62991727-9321http://hdl.handle.net/10400.1/14490Herbal beverages containing mixtures of L. algarvense (LA) flowers and C. sinensis (CS) were prepared and their inhibitory properties towards enzymes related with Alzheimer's (acetyl-and butyrylcholinesterase) and Type-2 diabetes mellitus (alpha-amylase and alpha-glucosidase) were evaluated for the first time. Samples were also appraised for antioxidant capacity and phenolic contents by high-performance liquid chromatography with diode-array detection (HPLC-DAD). Both synergistic and antagonistic interactions were observed: LA samples (infusion: IC50 = 0.22 mg/mL; decoction: 0.39 mg/mL) had higher acetylcholinesterase inhibition than CS and mixtures resulted in stronger enzymatic inhibition. CS had the highest rat alpha-glucosidase inhibition (infusion: IC50 = 3.91 mg/mL; decoction: IC50 = 2.50 mg/mL), which decreased when combined with LA. LA and CS samples had strong antioxidant activity, whereas LA and CS mixtures exhibited higher OH center dot radical-scavenging (0.56 <= SE <= 1.07) and anti-lipid peroxidation capacity (0.39 <= SE <= 0.81). CS had higher phenolic contents (infusion: 209 mg/g DW; decoction: 169 mg/g DW) and its combination with LA increased the phenolic diversity of the mixtures. Our results indicate that LA and CS infusions and decoctions and their combinations have relevant in vitro neuroprotective, antidiabetic and antioxidant properties and could be further explored as potential innovative functional beverages able to reduce the progression of neurodegenerative diseases and diabetic complications, and to prevent oxidative stress and lipid oxidation related diseases. (c) 2017 SAAB. Published by Elsevier B.V. All rights reserved.engKey enzymes relevantIn-vitroAntioxidant activityAlzheimers-diseaseDiabetes-mellitusAlpha-glucosidaseMedicinal-plantsConsumptionInfusionsPhenolicsjournal article10.1016/j.sajb.2017.12.003