Gonçalves, Rui A.Aragão, C.Frias, P. A.Sykes, António V.2014-07-032014-07-032012Gonçalves, R.A.; Aragão, C.; Frias, P.A.; Sykes, A.V.The use of different anaesthetics as welfare promoters during short-term human manipulation of European cuttlefish (Sepia officinalis) juveniles, Aquaculture, 370, s/n, 130-135, 20120044-8486http://hdl.handle.net/10400.1/4634Anaesthetics are frequently used to prevent injuries, stress and to promote welfare while handling animals. The efficiency of five anaesthetics for short-term handling of the European cuttlefish (Sepia officinalis) was tested in order to determine the best agent and its concentration. Ethanol (10.0, 20.0 and 30.0 mL.L−1 ), clove oil (0.05, 0.15 mL.L−1 ), hypothermia (8 °C), magnesium chloride (hexahydrated; 20.0, 27.0 g.L−1 ) and AQUI-S® (10.9 μl.L−1 ) were the selected anaesthetics. This choice was preceded by a preliminary trial, with additional agents, where 2-phenoxyethanol (10, 15, 20, 30 mL.L−1 ) and MS-222® (30, 40 mg.L−1 ) were used.eng2-PhenoxyethanolClove oilEthanol hypothermiaMagnesium chloridejournal article2014-06-27