Nowell, M. A.Power, DeborahGuerreiro, P. M.Llewellyn, LyndaRamsurn, Vimi P.Wigham, TrevorSweeney, Glen E.2014-10-212014-10-212000-03Mari A. Nowell, Deborah M. Power, Pedro M. Guerreiro, Lynda Llewellyn, Vimi Ramsurn, Trevor Wigham and Glen E. Sweeney. Cloning and Expression of an Elongation Factor-1a in Sea Bream (Sparus aurata) Larvae and Adult Tissue. Mar. Biotechnol. 2, 173–179, 20001436-2228AUT: DPO00386; PMG01122;http://hdl.handle.net/10400.1/5386A clone encoding the polypeptide elongation factor EF-1a was isolated from a complementary DNA library prepared from sea bream (Spartus aurata) larvae 1 to 10 days after hatching. The deduced amino acid sequence is between 82% and 95% similar to EF-1a in other animal species. EF-1a messenger RNA is present at low abundance in sea bream embryos prior to gastrulation, but at around 15 hours postfertilization, there is a 10-fold increase in transcript levels. This increase presumably reflects midblastula transition in this species. In adult sea bream, EF-1a appeared to have a relatively uniform distribution across all the tissues analyzed.engEF-1a cDNAExpressionSea beam (sparus aurata)TeleostLarvaeAdult tissuejournal articlehttp://dx.doi.org/ 10.1007/s101269900022