Rodriguez, A.Nunez, J.Nolasco, GustavoPonz, F.Sobrino, F.Deblas, C.2015-06-152015-06-1519940166-0934AUT: GNO00324;http://hdl.handle.net/10400.1/6228A PCR assay for the detection and characterization of foot-and-mouth disease virus was developed. The procedure allows RT-PCR amplification following direct adsorption of viral suspensions to microtiter plates, avoiding previous steps of phenol-extraction or heating. Using this procedure, FMDV-specific (based on 3D gene sequences), as well as serotype-specific (based on VP1 gene sequences) amplification were achieved for viral samples of serotypes A, O and C, either from cell culture supernatants or from lesions of infected animals. The assay allowed detection of around 15 PFU, being 500-fold more sensitive than a conventional indirect ELISA. This new method constitutes a simple, rapid and efficient alternative for the diagnosis and characterization of FMDV by PCR.engjournal articlehttps://dx.doi.org/10.1016/0166-0934(94)90030-2