Nóbrega, ClévioConceição, AndréCosta, Rafael GKoppenol, RebekahSequeira, Raquel L.Nunes, RicardoCarmo-Silva, SaraMarcelo, AdrianaMatos, Carlos ABetuing, SandrineCaboche, JocelyneCartier, NathalieAlves, Sandro2020-05-082020-05-082020-04-10BMC Research Notes. 2020 Apr 10;13(1):210http://hdl.handle.net/10400.1/13861Objective Compromised brain cholesterol turnover and altered regulation of brain cholesterol metabolism have been allied with some neurodegenerative diseases, including Huntington’s disease (HD). Following our previous studies in HD, in this study we aim to investigate in vitro in a neuroblastoma cellular model of HD, the effect of CYP46A1 overexpression, an essential enzyme in cholesterol metabolism, on huntingtin aggregation and levels. Results We found that CYP46A1 reduces the quantity and size of mutant huntingtin aggregates in cells, as well as the levels of mutant huntingtin protein. Additionally, our results suggest that the observed beneficial effects of CYP46A1 in HD cells are linked to the activation of autophagy. Taken together, our results further demonstrate that CYP46A1 is a pertinent target to counteract HD progression.engjournal article2020-05-01The Author(s)s13104-020-05053-x