Santos, Ana IsabelLourenco, Ana S.Simão, SóniaMarques-da-Silva, DorindaSantos, Daniela F.Carvalho, Ana Paula Onofre dePereira, Ana CatarinaIzquierdo-Álvarez, AliciaRamos, ElenaMorato, EsperanzaMarina, AnabelMartínez-Ruiz, AntonioAraújo, Inês2020-06-222020-06-222020-052213-2317http://hdl.handle.net/10400.1/14045Nitric oxide (NO) is well established as a regulator of neurogenesis. NO increases the proliferation of neural stem cells (NSC), and is essential for hippocampal injury-induced neurogenesis following an excitotoxic lesion. One of the mechanisms underlying non-classical NO cell signaling is protein S-nitrosylation. This post-translational modification consists in the formation of a nitrosothiol group (R-SNO) in cysteine residues, which can promote formation of other oxidative modifications in those cysteine residues. S-nitrosylation can regulate many physiological processes, including neuronal plasticity and neurogenesis. In this work, we aimed to identify S-nitrosylation targets of NO that could participate in neurogenesis. In NSC, we identified a group of proteins oxidatively modified using complementary techniques of thiol redox proteomics. S-nitrosylation of some of these proteins was confirmed and validated in a seizure mouse model of hippocampal injury and in cultured hippocampal stem cells. The identified S-nitrosylated proteins are involved in the ERK/MAPK pathway and may be important targets of NO to enhance the proliferation of NSC.engNitric oxideHippocampusS-nitrosylationSeizuresNeural stem cellsNeurogenesisjournal article10.1016/j.redox.2020.101457