Sroczynska, KatarzynaClaro, MariliaRange, PedroWasiak, KatarzynaWojtal-Frankiewicz, AdriannaBen-Hamadou, RadhouanLeitão, FranciscoChícharo, Luís2017-04-072017-04-0720160003-4088AUT: LCH00360;http://hdl.handle.net/10400.1/9701A modified flow-through chamber method was used to measure gross primary production (GPP), net primary production (NPP), community respiration (CR) and associated environmental variables in an intermittent Mediterranean-type stream in Southern Portugal. Three common types of in stream habitats were targeted: cobble (C), cobble covered with filamentous algae (C+A) and leaf litter (LL). NPP, GPP and CR differed significantly among all three habitats. GPP increased with chlorophyll a and, less strongly, with photosynthetic active radiation and, therefore, was highest in C+A habitat. The highest CR was in LL and its variation was best determined by ash-free dry mass (AFDM) of plant litter. Higher respiration in LL was related to heterotrophic activity and, to a lesser extent, to autotrophic respiration associated with periphyton. We observed a decrease of production efficiency of primary producers with AFDM in C+A and C habitats. Our results demonstrate that each habitat type should be considered as a discrete metabolic entity and that particular sets of environmental factors are responsible for habitat specific metabolic responses. Scaling up measurements from discrete habitat patches to the entire reach or stream should not be done by extrapolating the results of a single habitat type and will require quantification of habitat coverage, at the appropriate scale.engBenthic metabolismQuarteira River BasinLight and dark incubationsBenthic habitatsjournal article10.1051/limn/2016012