Rotllant, J.Ruane, N. M.Dinis, Maria TeresaCanario, Adelino V. M.Power, Deborah2014-05-152014-05-152006Rotllant, J.; Ruane, N. M.; Dinis, M. T.; Canario, A. V. M.; Power, D. M. Intra-adrenal interactions in fish: Catecholamine stimulated cortisol release in sea bass (Dicentrarchus labrax L.), Comparative Biochemistry and Physiology a-Molecular & Integrative Physiology, 143, 3, 375-381, 2006.1095-6433AUT: ACA00258; DPO00386;http://hdl.handle.net/10400.1/3948The effect of the catecholamines, adrenaline and noradrenaline, on sea bass (Dicentrarchus labrax) and sea bream (Sparus auratus) interrenal cortisol production was studied in vitro using a dynamic superfusion system technique. Increasing concentrations of catecholamines (10−6, 10−8 and 10−10 M) stimulated cortisol production in a dose-dependent manner, in sea bass only. The increase in cortisol production stimulated by adrenaline (10−6 M) and noradrenaline (10−6 M) was inhibited by sotalol (2×10−5 M), but not by prazosin suggesting that catecholamines stimulate cortisol release through the β-receptor subtype. To evaluate catecholamine-induced signal transduction in head kidney cells, measurements of cAMP production and [H3]myo-inositol incorporation were determined in head kidney cell suspensions. Adrenaline and noradrenaline (10−6 M) increased cAMP production, but had no effect on total inositol phosphate accumulation. These results indicate that catecholamines released from the chromaffin cells within the interrenal tissue may act as a paracrine factor to stimulate interrenal steroidogenesis in the sea bass.engCatecholaminesCortisolSparus auratusHead kidneySea bassDicentrarchus labraxGilthead sea breamjournal article2014-05-13http://dx.doi.org/10.1016/j.cbpa.2005.12.027