Esteves, E.Chicharo, AlexandraPina, T.Coelho, M. L.Andrade, José Pedro2013-02-212013-02-212000Esteves, E.; Chicharo, M. A.; Pina, T.; Coelho, M. L.; Andrade, J. P. Comparison of RNA/DNA ratios obtained with two methods for nucleic acid quantification in gobiid larvae, Journal of Experimental Marine Biology and Ecology, 245, 1, 43-55, 2000.0022-0981AUT: MCH00377;JAN00399;EES01759;http://hdl.handle.net/10400.1/2393In this paper, RNA and DNA contents and RNA/DNA ratios obtained with two fluorometric methods for the extraction and quantification of nucleic acids in individual estuarine gobiid larvae are compared. A total of 141 gobiid larvae, collected in the Mira and Guadiana estuaries (Portugal), were analysed. This study indicated that the nucleic acids content and RNA/DNA ratios of individual larvae varied with the procedure used. The two methods yielded similar log DNA values but different log RNA and log (RNA/DNA). The differences could be due to a number of factors related to specific steps of tested protocols. Comparison of nucleic acids contents and calibration of the two methods by regression analysis provided a reasonable basis for comparison although differences between estimates were evident.engFluorimetric methodsGobiid larvaeRNADNA ratiosMarine fish larvaeRNA-DNA ratiosjournal article2013-02-19