Geret, FlorenceBebianno, Maria João2020-05-082020-05-0820040147-6513http://hdl.handle.net/10400.1/13860Zinc is an essential trace element with many enzymatic functions that include antioxidant properties. To investigate whether an excess of Zn in the cells produces cytotoxicity or tissue damage or an imbalance in the antioxidant systems, marine clams (Ruditapes decussatus) were exposed to two sublethal Zn concentrations (100 and 1000 microg L(-1)) for 28 days. The effects of Zn on the activities of protective antioxidant enzymes (superoxide dismutase, catalase, and glutathione peroxidase, both total and selenium-dependent), lipid peroxidation, and metallothionein induction were followed in the gills and digestive gland of these clams. The results indicate that the effect of Zn exposure in this clam species depends not only on the tissue but also on the Zn concentration present. In the gills, catalase activity was enhanced by Zn exposure, whereas total glutathione peroxidase activity was inhibited. Lipid peroxidation occurred only in the clams exposed to the highest Zn concentration. In the digestive gland, the impact of Zn exposure on metabolic activity was less evident than in the gills. The most evident effect in both tissues was the enhancement of catalase activity by Zn exposure. Catalase and total glutathione peroxidase activities as well as lipid peroxidation are promising biomarkers to assess the effects of Zn in the gills of R. decussatus.engAnimalsAntioxidantsBiomarkersBivalviaCatalaseGillsGlutathione peroxidaseLipid peroxidationReactive oxygen speciesWater pollutantsZincEnvironmental exposureAntioxidant enzymesOxidative stressCellular zincMetallothioneinMetalMetabolismToxicityCadmiumDamagejournal article10.1016/j.ecoenv.2003.07.002