Butzke, Valéria Louzada LealFerreira, AliceOliveira Corrêa, Diego deFurlan, Júnior MendesGouveia, LuisaSchneider, Rosana de Cássia de SouzaCorbellini, Valeriano Antonio2024-09-032024-09-032024-06-190921-8971http://hdl.handle.net/10400.1/25825Microalgae are increasingly recognized as a valuable resource for bolstering sustainability in agriculture. However, current research and patents primarily focus on Chlorella spp., Scenedesmus spp., and Spirulina spp., thus leaving the vast diversity of microalgae relatively unexplored for agricultural applications. Euglena gracilis (Euglenophyta) is a microalga renowned for its resilience to diverse environmental stressors and capability to produce a variety of bioactive metabolites. This study investigated the potential of cultivating E. gracilis in piggery wastewater for nutrient recycling and as a source of beneficial biomolecules, particularly for biostimulant use. Utilizing raw wastewater diluted to 25% (P25) and pre-treated wastewater with photo-Fenton (PF), the research found that E. gracilis exhibited elevated cell density, biomass concentration, and overall cell health in both wastewaters compared to a synthetic medium (BG11-NPK). This was due to its efficient removal of nutrients, especially ammoniacal-nitrogen and phosphate, resulting in a biomass rich in polyunsaturated fatty acids, amino acids, and paramylon content.engCultivationSwine effluentNutrient recyclingBiochemical compositionSustainable agriculturejournal article10.1007/s10811-024-03286-y