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Estêvão, Maria Dulce da Mota Antunes de Oliveira
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- Cloning, characterisation and tissue distribution of an aquaporin-3 cDNA from fish (Sparus aurata)Publication . Santos, CRA; Fuentes, Juan; Cardoso, João CR; Estêvão, Dulce; Power, DeborahThe major intrinsic protein (MIP) family consists of several transmembrane channel proteins specific for water and neutral solutes. All proteins belonging to the MIP family evolved from two divergent bacterial paralogues, one giving rise to the CHIP group, functionally characterised as water channels and the other to the GLP group, specialised in glycerol transport. Three forms of MIP proteins belonging to the GLP group have been identified in mammals: aquaporin-3 (AQP-3), aquaporin-7 (AQP-7) and aquaporin-9 (AQP-9). We have recently cloned and characterised a GLP cDNA from the marine teleost sea bream (Sparus aurata) and studied its tissue distribution. Phylogenetic analysis revealed it was most like AQP-3 and further studies are now underway to determine its role in hydromineral balance.
- Effects of body weight regain on leptin levels: a systematic review and meta-analysisPublication . Rostami Rayeni, Najme; Abdollahzad, Hadi; Alibakhshi, Pooya; Morvaridzadeh, Mojgan; Heydari, Hafez; Dehnad, Afsaneh; Khorshidi, Masoud; Izadi, Azimeh; Shidfar, Farzad; Estêvão, Dulce; Omidi, Amirhosein; Heshmati, JavadBackground: There are different changes observed before and after diet therapy, and also after weight regain. However, there is not sufficient information regarding weight regain and hormonal changes. Purpose: The purpose of this study was to review the connection between weight regain and leptin concentration levels. Methods: MEDLINE, SCOPUS, Web of Science, and the Cochrane Library were searched for interventional articles published from January 1, 1980, to June 30, 2020. Randomized clinical trials with parallel or cross over design assessing leptin concentrations at the baseline and at the end of study were reviewed. Two independent reviewers extracted data related to study design, year of publication, country, age, gender, body mass index (BMI), duration of the following up period and mean +/- SD of other intended variables. Results: Four articles were included, published between 2004 and 2016. Three of them were conducted in the US and one of them in Netherland. Sample size of the studies ranged between 25 and 148 participants. The range of following up period was from13 to 48 weeks. The age range of participants was from 34 to 44 years. Our analysis shows that weight regain could reduce leptin levels, but this change is not statistically significant. Conclusion: This review suggests that weight regain may induce a non-significant reduction in leptin level. However, the limited number and great heterogeneity between the included studies may affect the presented results and there are still need to well-designed, large population studies to determine the relationship between weight regain and leptin levels.
- Tissue responsiveness to estradiol and genistein in the sea bass liver and scalePublication . Estêvão, M. Dulce; Andrade, André; Santos, Soraia; Power, Deborah; Pinto, Patricia ISAs in mammals, estrogens in fish are essential for reproduction but also important regulators of mineral homeostasis. Fish scales are a non-conventional target tissue responsive to estradiol and constitute a good model to study mineralized tissues effects and mechanisms of action of estrogenic compounds, including phytoestrogens. The responsiveness to estradiol and the phytoestrogen genistein, was compared between the scales and the liver, a classical estrogenic target, in sea bass (Dicentrarchus labrax). Injection with estradiol and genistein significantly increased circulating vitellogenin (for both compounds) and mineral levels (estradiol only) and genistein also significantly increased scale enzymatic activities suggesting it increased mineral turnover. The repertoire, abundance and estrogenic regulation of nuclear estrogen receptors (ESR1, 2a and 2b) and membrane G-protein receptors (GPER and GPER-like) were different between liver and scales, which presumably explains the tissue-specific changes detected in estrogen-responsive gene expression. In scales changes in gene expression mainly consisted of small rapid increases, while in liver strong, sustained increases/decreases in gene expression occurred. Similar but not overlapping gene expression changes were observed in response to both estradiol and genistein. This study demonstrates for the first time the expression of membrane estrogen receptors in scales and that estrogens and phytoestrogens, to which fish may be exposed in the wild or in aquaculture, both affect liver and mineralized tissues in a tissue-specific manner. (C) 2015 Elsevier Ltd. All rights reserved.
- Consumo de medicamentos no internamento dos Serviços Clínicos de Psiquiatria e Saúde Mental e NeurologiaPublication . Lavaredas, Ana; Nascimento, Tânia; Estêvão, DulceNa realidade que a nossa sociedade vive actualmente, cada vez mais há patologias que envolvem Sistema Nervoso Central: transtornos da ansiedade generalizada e do pânico, comportamentos obsessivo-compulsivos, epilepsia, doença de Parkinson, cefaleias, demências, entre outros. O aumento da esperança média de vida leva a que incidência e prevalência esteja a agravar-se. Este trabalho teve como objectivo determinar os dez medicamentos mais consumidos durante um mês (Novembro de 2009), nos serviços clínicos (SC) de Psiquiatria e Saúde Mental e de Neurologia de um hospital da grande Lisboa, e perceber a relação entre estes consumos e as patologias mais frequentes nestes serviços.
- Efeitos de fitoestrogénios no metabolismo mineral em escamas de robalo e de tilápia moçambicanaPublication . Estêvão, Dulce; Pinto, Patricia IS; Santos, Soraia; Andrade, André; Power, DeborahO rápido desenvolvimento da aquacultura nas últimas décadas fez aumentar a procura por fontes proteicas adequadas para incluir nas rações dos peixes. A soja tem sido muito utilizada com fonte proteica de origem vegetal mas é particularmente rica em fitoestrogénios, incluindo a genisteína (GEN) e a daidzeína (DAI), que são as principais isoflavonas presentes na soja. Os peixes podem estar expostos aos fitoestrogénios no ambiente ou através das dietas que os contêm, como é o caso da soja. Estes compostos podem ter atividades estrogénicas e efeitos disruptivos na reprodução mas o seu impacto nos tecidos mineralizados continua a ser desconhecido. As escamas de peixe são um tecido mineralizado que, tal como o osso de mamíferos, é mantido por ciclos de formação e reabsorção, mediado por osteoblastos (OSB) e osteoclastos (OSC), respetivamente. As escamas são um tecido responsivo aos estrogénios e expressam os recetores de estrogénio nucleares (ERs). As atividades das enzimas fosfatase alcalina (ALP) e fosfatase ácida resistente ao tartrato (TRAP) são usadas como marcadores das atividades dos OSB e OSC, respetivamente, e são modificadas pelo estradiol (E2) nas escamas de várias espécies de peixe. Usando um ensaio in vitro, investigámos o possível impacto da exposição a GEN e a DAI no metabolismo mineral em escamas. O efeito destes compostos foi avaliado através da determinação das atividades de TRAP e ALP em escamas de robalo (Dicentrarchus labrax), uma espécie marinha, e de tilápia moçambicana (Oreochromis mossambicus), mantida em água salgada (AS) e em água doce (AD).
- Effect of saffron supplementation on oxidative stress parameters: A systematic review and meta‐analysis of randomized placebo‐controlled trialsPublication . Morvaridzadeh, Mojgan; Agah, Shahram; Estêvão, M. Dulce; Hosseini, Ava Sadat; Heydari, Hafez; Toupchian, Omid; Abdollahi, Shima; Persad, Emma; Abu‐Zaid, Ahmed; Rezamand, Gholamreza; Heshmati, JavadOxidative stress (OS), the absence of equilibrium between prooxidants and antioxi dants in the body, has been shown to play a pivotal role in the initiation and pro gression of many diseases. Saffron has been noted for its antioxidant capacity and can be used to improve OS parameters in unhealthy patients. Our aim was to evalu ate the efficacy of saffron supplementation on OS parameters in unhealthy patients in randomized controlled trials (RCTs). We searched Medline, EMBASE, Cochrane CENTRAL, Scopus, and Web of Science without language restrictions for RCTs up until April 2021. Studies were included if they compared any form of saffron sup plementation to placebo or no supplementation on OS parameters in unhealthy pa tients. Using a random-effects model with calculated standardized mean difference (SMD) and 95% confidence intervals (CI), we quantitatively synthesized the data. Heterogeneity was assessed using Cochrane's I 2 values. Ten randomized controlled trials were eligible for this review. Seven were included in the meta-analysis and indicated an association between saffron intake and a statistically significant decrease in malondialdehyde (MDA) levels (SMD: −0.40; 95% CI: −0.63, −0.17; I 2 = 32.6%) and a significant increase in total antioxidant capacity (TAC, SMD: 0.24; 95% CI: 0.05, 0.42; I 2 = 00.0%). Saffron intake was shown to significantly impact MDA and TAC, indicating its beneficial properties in improving OS in unhealthy patients. However, additional RCTs are required to evaluate the effect on other OS parameters.
- The volunteer functions inventory (VFI): Adaptation and psychometric properties among a portuguese sample of volunteersPublication . Martins, Cátia; da Silva, José Tomás; Jesus, Saúl; Ribeiro, Conceição; Estêvão, M. Dulce; Baptista, Ricardo; Carmo, Cláudia; Brás, Marta; Santos, Rita; Nunes, C.: The Volunteer Functions Inventory (VFI) is an instrument widely used to assess volunteers’ motivation based on the Functionalist Model of Omoto and Snyder. It assesses six factors that reflect several motivational functions. The VFI has been translated into various languages and validated in different cultural contexts, but some studies have reported different factor structures (e.g., five or four factors). In the Portuguese context, previous studies have also shown inconsistent results. The aim of this study was to adapt and validate the VFI for Portuguese volunteers, testing several alternative models (nine models) using confirmatory factor analysis. The sample comprised 468 volunteers (76.3% women), aged from 13 to 81 years (M = 36.66, SD = 14.93). The results support the original interrelated six-factor model as the best-fitting one. The VFI showed good internal consistency and convergent validity. Significant correlations were found between the VFI factors, organizational commitment, and volunteers’ satisfaction. Overall, the six-factor VFI is a valid and reliable tool for measuring the motivational functions of Portuguese volunteers, with implications for practice and research in the volunteering field.
- Effect of maternal restricted diet during late gestation on muscle and bone development in sheep offspringPublication . Estêvão, Dulce; Harrison, Adrian; McKenzie, S. H.; Ribeiro, Luís Pedro; Tygesen, M. P.; Sancho, Teresa; Power, DeborahChanges in intrauterine environment, including nutrient availability, have been associated with fetal programming, contributing to different phenotypes which may determine health and susceptibility to disease throughout life. These changes seem to be mediated through alterations in both anabolic and catabolic hormone levels of maternal, placental and/or fetal origin. The present work aimed to evaluate how maternal under-nutrition during late pregnancy affects muscle and bone growth. Pregnant ewes were divided into two groups, one fed ad libitum and the other fed a restricted diet (50% of total energy requirements) during the last 6 weeks of gestation. Three twin carrying ewes from each feeding group were euthanized 6 days pre parturition. The remaining ewes gave birth normally and reared their lambs. At approximately day 30 post partum, 5 lambs from each of the feeding groups were euthanized and samples collected. Nutrient restriction during late gestation did not affect intrauterine axial growth, although weight at birth and the muscle weight were significantly lower than the ad libitum fed lamb fetuses. Bone development is less affected cf muscle development during periods of maternal feed restriction; however, catch-up growth of muscle occurs when lambs (30 days post-parturition) have access to adequate rations. In utero irrespective of maternal nutrient supply dry muscle mass is correlated (r=0.94) to bone development (bone weight, femur length and femur mineral density). In contrast, post partum growth of and skeleton are less tightly coupled and unaffected by the events in utero. A detailed examination of how maternal nutrient supply affects endocrine parameters in utero will be required to assess if it affects susceptibility post-partum to endocrine dysfunction.
- Effect of synbiotic bread containing lactic acid on blood lipids and apolipoproteins in patients with type 2 diabetes: A randomized controlled trialPublication . Ghafouri, Atie; Heshmati, Javad; Heydari, Iraj; Shokouhi Shoormasti, Raheleh; Estêvão, M. Dulce; Hoseini, Ava Sadat; Morvaridzadeh, Mojgan; Akbari‐Fakhrabadi, Maryam; Farsi, Farnaz; Zarrati, Mitra; Pizarro, Ana Beatriz; Shidfar, Farzad; Ziaei, SomayehRecently, the use of synbiotics for managing various diseases has dramatically increased. Synbiotics have been shown to be a good approach to influence the composition of the gut microbiota with positive health effects. Management of type 2 diabetes mellitus (T2DM) complications is one of the reasons for the ingestion of synbiotics and so the aim of the current study was to determine the effects of synbiotic bread intake on markers of lipid profile in T2DM patients. One hundred T2DM patients (age between 20 and 60 years) were randomly assigned to four groups to consume different types of synbiotic bread, three times/day, for 8 weeks: "synbiotic + lactic acid" (n = 25; IV), "synbiotic" (n = 25; III), "lactic acid brad" (n = 25; II), or "control" (a = 25; I). The measured outcomes included anthropometric characteristics, glycemic control parameters, blood lipids, and apolipoproteins. The consumption of "synbiotic + lactic acid bread" (group IV) and "lactic acid bread" (group II) led to a significant decrease in total cholesterol (TC) and glycated hemoglobin (HbA1c) compared to the "control bread." The HbA1c levels were also significantly lower when compared to group II. Additionally, apolipoprotein A (Apo A1) levels were significantly decreased in group IV, compared to control and other groups (post hoc analysis). No significant differences between groups were observed for triglyceride (TG), high-density lipoprotein (HDL), low-density lipoprotein (LDL), and apolipoprotein B100 (Apo B100) levels. The observed results show that the synbiotic bread (with or without lactic acid) promoted a decrease in total cholesterol (TC) and Apo A1 in diabetic patients when consumed daily for 8 weeks.
- Caso de polimedicação numa farmácia comunitáriaPublication . Sousa, S. S.; Estêvão, Dulce; Nascimento, T.O envelhecimento é um processo dinâmico e progressivo no qual ocorrem alterações morfológicas, funcionais e bioquímicas, resultando numa diminuição da capacidade de regulação do organismo, necessária à manutenção de uma condição estável, em situações de sobrecarga funcional, tais como a administração de medicamentos. Em indivíduos idosos existe uma maior prevalência de doenças crónicas, o que requer uma maior e mais específica prestação de cuidados de saúde. Por outro lado, a multiplicidade de doenças crónicas leva à prescrição de vários fármacos ao doente e, por consequência, ao aumento do risco de interacções farmacológicas e reacções adversas inerentes. A polimedicação define-se como a utilização de vários medicamentos, prescritos e/ou de automedicação, que sendo eventualmente, desnecessários, podem causar reacções adversas e/ou interacções medicamentosas, que aumentam consoante o número de medicamentos administrados. Os riscos associados polimedicação mostram que, tanto em idosos como em qualquer outra faixa etária, a terapêutica farmacológica deve ser eficaz, segura e racional, no sentido de evitar resultados negativos da medicação.