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Functional expression of the human CHIP28 water channel in a yeast secretory mutant

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Abstract(s)

The temperature-sensitive Saccharomyces cerevisiae mutant strain NY17, deficient in the secretory pathway (sec6-4 mutation), is used for the heterologous expression of the human CHIP28 water channel. After a heat-shock, the protein is present in partially purified post-golgi secretory vesicles, lmmunodetection and water transport studies, directly made on the vesicles, showed that CHIP28 is highly expressed and active in the yeast membranes.

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Keywords

Saccharomyces cerevisiae sec6-4 nutant Post-golgi secretory vesicles Water transport AQP1

Citation

Laizé V; Rousselet G; Verbavatz JM; Berthonaud V; Gobin R; Roudier N; Abrami L; Ripoche P; Tacnet F. Functional expression of the human CHIP28 water channel in a yeast secretory mutant, FEBS Letters, 373, 3, 269-274, 1995.

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